A C-type lectin with a single CRD from Onychostoma macrolepis mediates immune recognition against bacterial challenge

C-type lectins (CTL) are a large group of pattern-recognition proteins and to play important roles in glycoprotein metabolism, multicellular integration, immunity. Based on their overall domain structure, they can be classified as different groups that possess physiological functions. A typical lectin (named OmLec1) was identified from the fish, Onychostoma macrolepis, an cultured fish China. Open reading frame OmLec1 contains 570 bp, encoding protein 189 amino acids includes signal peptide single carbohydrate-recognition domain. The phylogenetic analysis showed could grouped with other fish. expressed all tissues our study, expression level highest liver. And its relative levels were significantly upregulated following infection Aeromonas hydrophila. recombinant (rOmLec1) agglutinate some Gram-negative bacteria Gram-positive vitro presence Ca2+, showing Ca2+-dependent carbohydrate-binding protein. Furthermore, rOmLec1 purified E. coli BL21 (DE3), strongly bound LPS PGN, well tested manner. These results indicate plays central role innate immune response pattern recognition receptor recognizes diverse pathogens among O. macrolepis.